Taw qhia

Nyob rau hauv niaj hnub biomedical thiab chemical laboratories,Cov kab lis kev cai pov tseg tau siv dav hauv cov kab lis kev cai ntawm tes, tshuaj lom neeg cov tshuaj tiv thaiv, cov qauv khaws cia thiab lwm yam haujlwm tseem ceeb.Lawv qhov tseem ceeb hauv cov txheej txheem sim tsis tuaj yeem tsis quav ntsej. Txij li cov kab lis kev cai no nyob rau hauv kev sib cuag ncaj qha nrog cov qauv kev sim, lawv cov khoom siv, qhov loj me, kaw thiab txawm tias lawv tau ua kom tsis muaj menyuam lossis tsis tuaj yeem muaj feem cuam tshuam rau qhov kev sim. Kev xaiv tsis raug yuav ua rau muaj kev sib kis kab mob, cov qauv poob lossis kev sim cov ntaub ntawv tsis ncaj ncees, yog li cuam tshuam rau qhov raug thiab rov tsim dua ntawm txoj kev tshawb no.

Yam Tseem Ceeb Ntawm Cov Kab Tsuag Kab Tsuag

Muaj ntau hom kab lis kev cai pov tseg, thiab cov kws tshawb fawb yuav tsum xaiv hom zoo raws li lub hom phiaj ntawm kev sim, qhov chaw ua haujlwm thiab cov yam ntxwv ntawm cov qauv. Lawv tau muab faib ua peb yam hauv qab no: cov khoom siv, muaj peev xwm thiab kev ua haujlwm tshwj xeeb:

1. Kev faib tawm los ntawm cov khoom siv

Cov kab lis kev cai pov tseg ua los ntawm cov khoom sib txawv sib txawv hauv qhov kub thiab txias, tshuaj lom neeg ruaj khov thiab cov khoom siv kho qhov muag:

• Polypropylenee: Zoo tiv taus kub thiab tshuaj lom neeg corrosion, haum rau niaj hnub cell kab lis kev cai, molecular biology thwmsim thiab lwm yam kev siv.
• Polystyrene: siab transparency, yooj yim los soj ntsuam cov kua thiab lub xeev ntawm tes, feem ntau siv nyob rau hauv optical kuaj, tab sis tsis kub kub kuj, feem ntau tsis tuaj yeem siv rau autoclaving.
• iav kab lis kev cai raj: Txawm hais tias rov siv tau thiab muaj tshuaj lom neeg ruaj khov, lawv raug nqi kim heev, yuav tsum muaj cov txheej txheem ntxuav thiab ua kom tsis muaj menyuam ntxiv, thiab nthuav tawm qhov kev pheej hmoo ntawm kev sib kis.

2. Kev faib tawm los ntawm kev muaj peev xwm

Raws li qhov ntim ntawm cov qauv xav tau rau qhov kev sim, lub peev xwm ntawm cov kab lis kev cai yog li ntawm micro mus rau qhov ntim loj:

• Microcentrifuge tubes: feem ntau yog siv rau cov qauv xa tawm, centrifugal nag lossis daus, DNA / RNA rho tawm thiab lwm yam haujlwm.
• Standard kab lis kev cai raj: lub peev xwm siv ntau tshaj plaws hauv chav kuaj, haum rau kab lis kev cai ntawm tes, sib xyaw cov tshuaj tiv thaiv, qauv khaws cia thiab lwm yam.
• Loj-muaj peev xwm kab lis kev cai raj: haum rau loj-scale kab lis kev cai los yog loj npaum li cas ntawm kev daws teeb meem.

3. Kev faib tawm los ntawm kev ua haujlwm tshwj xeeb

Cov raj muaj nyob nrog ntau yam ntxiv kom tau raws li cov kev xav tau tshwj xeeb:

• Aseptic Pre-sterilized Tubes: Hoobkas sterilized los ntawm gamma hluav taws xob los yog autoclaving, haum rau kev sim nrog siab aseptic yuav tsum tau.
• Nrog cartridge cap: tso cai rau kev sib pauv roj, haum rau cov kab mob los yog cov kab mob ntawm tes uas xav tau aeration thiab tiv thaiv kev kis kab mob los ntawm exogenous qhov chaw.
• Tsawg Kub Resistant Tubes: tuaj yeem siv tau zoo nyob rau hauv -80 ℃ lossis txawm tias ib puag ncig ua kua nitrogen, tsim nyog rau lub sijhawm ntev ntawm kev khaws cia ntawm cov qauv lom.
• Cov tub ntxhais kawm tiav / tsis kawm tiav: Cov tub ntxhais kawm tiav tau yooj yim rau kev kwv yees ceev thiab xa cov kua ntim los txhim kho kev ua haujlwm ntawm kev sim.

Yam tseem ceeb hauv kev xaiv kab lis kev cai

Nyob rau hauv tus txheej txheem ntawm kev sim tsim thiab kev ua haujlwm, kev xaiv cov kab lis kev cai pov tseg uas tsim nyog yog qhov tseem ceeb los xyuas kom meej qhov kev sim ua tau zoo thiab qhov tseeb ntawm cov txiaj ntsig. Cov kws tshawb fawb yuav tsum ua tib zoo xav txog ntau yam tseem ceeb:

1. Hom kev sim

Cov kev xav tau rau cov kab lis kev cai sib txawv los ntawm kev sim mus rau kev sim thiab yuav tsum tau xaiv raws li cov ntsiab lus ntawm kev ua haujlwm thiab qhov chaw sim:

• Rau cell kab lis kev cai: Cov kev cai rau kev ua kom tsis muaj menyuam yog qhov siab heev, thiab kev siv cov raj ua kom tsis muaj menyuam ua ntej uas muaj cov pa roj carbon monoxide caps kom ntseeg tau tias kev sib pauv roj tau pom zoo.
• PCR / molecular biology kev sim: yuav tsum tsis pub muaj DNA enzymes, RNA enzymes thiab pyrogen-dawb tubing, feem ntau siv cov tubing huv-qib polypropylene.
• Tsawg kub cia: Cov raj uas muaj qhov kub tsis zoo yuav tsum tau siv kom tsis txhob muaj lysis ntawm qhov kub thiab txias.

2. Qauv yam ntxwv

Lub zog physicochemical ntawm tus qauv ncaj qha cuam tshuam rau kev xaiv cov khoom siv thiab kev ua haujlwm ntawm cov kab lis kev cai raj:

• Cov kua dej los yog cov khoom kuaj: txiav txim siab qhov yuav tsum tau ntim thiab daim ntawv ntawm lub raj orifice.
• Acid los yog alkaline kuaj: Cov qauv corrosive heev xav tau cov khoom siv tshuaj tiv thaiv kom tsis txhob muaj cov raj deformation los yog paug.
• Seb puas yuav zam lub teeb: Cov qauv teeb pom kev zoo yuav tsum xaiv cov khoom siv amber lossis opaque khoom kab lis kev cai raj, txhawm rau tiv thaiv photodegradation.

3. Sterilization yuav tsum muaj

Qhov xav tau rau kev ua kom tsis muaj menyuam thiab hom kev ua kom tsis muaj menyuam yog qhov tseem ceeb hauv kev xaiv cov tubing:

• Pre-sterilization vs. self-sterilization: Hoobkas pre-sterilized khoom yog tsim rau high-throughput thwmsim, txuag lub sij hawm; Yog tias lub chaw kuaj mob muaj cov khoom siv autoclave kom tsis muaj menyuam, koj tuaj yeem xaiv PP tubing uas tuaj yeem ua autoclaved.
• Sterilization compatibility: Piv txwv li, cov khoom siv PS tsis haum rau autoclaving thiab tsuas yog siv rau ib leeg xwb.

4. Kev sib haum xeeb

Cov hlab ntsha yuav tsum tau ua kom zoo rau cov khoom siv hauv chav kuaj kom ntseeg tau tias kev ua haujlwm zoo thiab txhim khu kev qha:

• Centrifugal compatibility: Cov raj siv rau centrifugation yuav tsum muaj cov txheej txheem muaj zog kom tiv taus centrifugal rog ntawm kev sib hloov ceev.
• Automation compatibility: Rau cov kev sim uas yuav tsum tau siv cov pipetting robots, automated dispensing systems, thiab lwm yam, yuav tsum tau siv cov qauv tube loj.

5. Nqi thiab sustainability

Kev tswj hwm tus nqi tsim nyog thiab kev siv peev txheej kuj yog ib qho tseem ceeb hauv kev ua kom tau raws li kev xav tau ntawm kev sim:

• Pov tseg vs. reusable: cov raj uas siv tau yooj yim los tswj thiab zam kom tsis txhob kis kab mob, haum rau kev sim siab dhau los; reusable iav raj yog tsim rau kev sim yooj yim nrog kev siv nyiaj tsawg.
• Muas scale: Cov khoom ntim loj tuaj yeem txo cov nqi tsev, haum rau lub sijhawm ntev, cov haujlwm loj; me me, customized specifications yog ntau saj zawg zog tab sis kuj tus nqi siab.

Cov lus pom zoo rau Cov Ntawv Thov Kev Sib Tham

Raws li cov kev xav tau tshwj xeeb ntawm ntau hom kev sim, cov kab lis kev cai pov tseg hauv qab no tau pom zoo rau ntau qhov kev thov, txhawm rau txhim kho kev ua tau zoo thiab kev ntseeg siab ntawm kev sim:

1. Kev cog qoob loo ntawm tes

• Hom kev pom zoo: sterile polypropylene kab lis kev cai raj nrog breathable cartridge caps
• Yog vim li cas: Cov khoom siv polypropylene muaj cov tshuaj zoo inertness thiab biocompatibility, haum rau cell kab lis kev cai kua. Lub npog ntsej muag tuaj yeem paub txog kev sib pauv roj zoo, tiv thaiv cov kab mob microbial, thiab ua tau raws li cov pa ib puag ncig uas xav tau rau kev loj hlob ntawm tes.

2. PCR/qPCR molecular realization

• Pom zoo hom: Nuclease-dawb, pyrogen-dawb tshwj xeeb PCR hlab lossis microcentrifuge hlab
• Yog vim li cas: High purity polypropylene hlab, tom qab kev kho mob nruj, tuaj yeem zam cov qauv degradation los yog paug, kom ntseeg tau tias qhov tseeb thiab txhim khu kev qha amplification. Nyias-phab ntsa tsim yog pom zoo los txhim kho cov cua sov hloov efficiency.

3. Tsawg kub cia

• Hom kev pom zooTug: Tsis tshua muaj kub Resistant Polypropylene Tubing nrog Anti-Freeze Cracking Tsim thiab Ntsia Hlau Foob Cap
• Yog vim li cas: Cov raj no tuaj yeem siv los khaws cov qauv rau lub sijhawm ntev ntawm -80 ° C lossis txawm nyob hauv cov kua nitrogen, thiab cov qauv tshwj xeeb tiv thaiv lub raj los ntawm kev tawg thiab cov qauv xau. Haum rau kev khaws cia ntev ntawm cov kab ntawm tes, kuaj ntshav, proteins lossis nucleic acids.

4. Centrifuge

• Pom zoo hom: siab kam rau ua polypropylene raj, puag ncig los yog conical hauv qab tsim, nrog centrifuge rotors
• Yog vim li cas: PP raj muaj qhov zoo centrifugal tsis kam thiab tuaj yeem tiv taus siab centrifugal rog yam tsis muaj deformation lossis tawg. Lub conical hauv qab pab khaws cov hlwb los yog precipitates centrally thiab nce tus nqi rov qab.

Feem ntau yuam kev thiab txoj hauv kev kom zam tau lawv

Hauv kev xyaum, kev sim ua tsis tiav vim xaiv cov kab lis kev cai tsis raug tshwm sim los ntawm lub sijhawm. Ntau qhov ua yuam kev thiab cov lus qhia sib xws rau cov kev daws teeb meem tau teev tseg hauv qab no rau cov kws tshawb fawb siv:

1. Kev siv cov ntaub ntawv tsis-siab kub resistant rau autoclaving