Kev Taw Qhia

Hauv cov chaw kuaj mob biomedical thiab tshuaj lom neeg niaj hnub no,Cov raj xa kab lis kev cai pov tseg tau siv dav hauv kev cog qoob loo ntawm tes, cov tshuaj lom neeg, kev khaws cia cov qauv thiab lwm yam haujlwm tseem ceeb.Lawv qhov tseem ceeb hauv cov txheej txheem sim tsis tuaj yeem tsis quav ntsej. Vim tias cov raj kab lis kev cai no sib cuag ncaj qha nrog cov qauv sim, lawv cov khoom siv, qhov loj me, kev kaw thiab txawm tias lawv puas tau sterilized lossis tsis tau yuav muaj kev cuam tshuam loj rau cov txiaj ntsig ntawm kev sim. Kev xaiv tsis raug yuav ua rau muaj kev sib kis, poob cov qauv lossis kev ntxub ntxaug cov ntaub ntawv sim, yog li cuam tshuam rau qhov tseeb thiab kev rov ua dua ntawm kev tshawb fawb.

Cov Hom Phiaj Tseem Ceeb ntawm Cov Raj Mis Siv Ib Zaug Xwb

Muaj ntau hom raj siv pov tseg, thiab cov kws tshawb fawb yuav tsum xaiv hom zoo raws li lub hom phiaj ntawm kev sim, qhov chaw ua haujlwm thiab cov yam ntxwv ntawm cov qauv. Lawv raug faib ua peb yam hauv qab no: cov khoom siv, lub peev xwm thiab kev ua haujlwm tshwj xeeb:

1. Kev faib tawm los ntawm cov khoom siv

Cov raj xa dej pov tseg uas ua los ntawm cov khoom sib txawv sib txawv hauv kev tiv taus kub, kev ruaj khov tshuaj lom neeg thiab cov khoom siv kho qhov muag:

• Polypropylenee: Zoo tiv taus kub thiab tshuaj lom neeg corrosion, tsim rau kev coj noj coj ua ntawm tes, kev sim molecular biology thiab lwm yam kev siv.
• Polystyrene: pob tshab siab, yooj yim los saib cov kua thiab lub xeev cell, feem ntau siv rau hauv kev sim kho qhov muag, tab sis tsis kub siab tiv taus, feem ntau tsis tuaj yeem siv rau autoclaving.
• Cov raj iav kab lis kev caiTxawm hais tias siv tau dua thiab ruaj khov hauv tshuaj lom neeg los xij, lawv kim heev, xav tau kev ntxuav thiab tua kab mob ntxiv, thiab muaj kev pheej hmoo ntawm kev sib kis kab mob.

2. Kev faib tawm los ntawm lub peev xwm

Nyob ntawm seb qhov ntim ntawm cov qauv uas xav tau rau qhov kev sim, lub peev xwm ntawm cov raj kab lis kev cai yog txij li me me mus rau qhov ntim loj:

• Cov raj xa dej me me: feem ntau siv rau kev faib cov qauv, centrifugal precipitation, DNA / RNA extraction thiab lwm yam haujlwm.
• Cov raj xa kab lis kev cai txheem: lub peev xwm siv ntau tshaj plaws hauv chav kuaj, tsim nyog rau kev cog qoob loo ntawm tes, kev sib tov tshuaj, kev khaws cia qauv thiab lwm yam hom phiaj.
• Cov raj xa dej loj muaj peev xwm loj: tsim nyog rau kev loj hlob ntawm tes lossis ntau cov txheej txheem daws teeb meem.

3. Kev faib tawm los ntawm kev ua haujlwm tshwj xeeb

Cov raj muaj ntau yam ntxiv kom tau raws li qhov xav tau tshwj xeeb:

• Cov raj mis aseptic ua ntej sterilized: Hoobkas sterilized los ntawm gamma hluav taws xob lossis autoclaving, tsim rau kev sim nrog cov kev xav tau aseptic siab.
• Nrog lub hau cartridge: tso cai rau kev sib pauv roj, tsim nyog rau cov kab mob me me lossis cov kab cell uas xav tau aeration thiab tiv thaiv kev ua qias tuaj ntawm cov chaw sab nraud.
• Cov raj tiv taus kub tsawg: siv tau zoo hauv -80 ℃ lossis txawm tias kua nitrogen ib puag ncig, tsim nyog rau kev khaws cia ntev ntawm cov qauv tshuaj lom neeg.
• Cov raj xa dej uas muaj qib kawm tiav/tsis muaj qib kawm tiav: Cov raj xa dej uas tau kawm tiav lawm yooj yim rau kev kwv yees sai thiab faib cov kua dej kom txhim kho kev ua haujlwm ntawm kev sim.

Cov Yam Tseem Ceeb Hauv Kev Xaiv Cov Raj Kab Lis Kev Cai

Thaum lub sijhawm tsim qauv thiab ua haujlwm sim, kev xaiv cov raj xa dej uas siv pov tseg yog qhov tseem ceeb heev kom ntseeg tau tias kev sim ua haujlwm tau zoo thiab raug. Cov kws tshawb fawb yuav tsum xav txog ntau yam tseem ceeb:

1. Hom kev sim

Cov kev cai rau cov raj xa dej sib txawv ntawm kev sim mus rau kev sim thiab yuav tsum tau xaiv raws li cov ntsiab lus ntawm kev ua haujlwm thiab qhov chaw sim:

• Rau kev cog qoob loo ntawm tes: qhov yuav tsum tau ua kom tsis muaj menyuam yog qhov siab heev, thiab siv cov raj xa dej uas tau ua kom tsis muaj menyuam ua ntej nrog cov hau cartridge uas ua pa tau kom ntseeg tau tias muaj kev sib pauv roj.
• Kev sim PCR / molecular biology: yuav tsum tsis muaj DNA enzymes, RNA enzymes thiab pyrogen-free tubing, feem ntau siv cov polypropylene tubing huv.
• Kev khaws cia kub tsawg: Cov raj uas muaj qhov kub thiab txias tsis zoo yuav tsum tau siv kom tsis txhob lysis ntawm qhov kub thiab txias qis.

2. Cov yam ntxwv ntawm cov qauv

Cov khoom siv physicochemical ntawm cov qauv cuam tshuam ncaj qha rau kev xaiv cov khoom siv thiab kev teeb tsa ua haujlwm ntawm cov raj kab lis kev cai:

• Cov qauv kua los yog khoom khov: txiav txim siab qhov ntim xav tau thiab daim ntawv ntawm lub raj orifice.
• Cov qauv kua qaub lossis alkaline: cov qauv corrosive heev xav tau cov ntaub ntawv tiv taus tshuaj lom neeg kom tsis txhob muaj lub raj deformation lossis kev ua qias tuaj.
• Seb puas yuav zam lub teeb: cov qauv uas rhiab rau lub teeb yuav tsum xaiv cov raj xa kab lis kev cai amber lossis opaque, txhawm rau tiv thaiv kev puas tsuaj ntawm photodegradation.

3. Cov kev cai rau kev ua kom huv si

Qhov xav tau kev tua kab mob thiab hom kev tua kab mob siv yog qhov tseem ceeb uas yuav tsum xav txog thaum xaiv cov raj:

• Kev ua kom huv ua ntej piv rau kev ua kom huv tus kheej: cov khoom lag luam ua ntej sterilized Hoobkas yog qhov tsim nyog rau kev sim siab-throughput, txuag lub sijhawm; yog tias lub chaw kuaj mob muaj cov khoom siv autoclave sterilization, koj tuaj yeem xaiv PP tubing uas tuaj yeem autoclaved.
• Kev sib xws ntawm kev ua kom tsis muaj menyuamPiv txwv li, cov khoom siv PS tsis haum rau autoclaving thiab tsuas yog siv ib zaug xwb.

4. Kev Sib Haum Xeeb

Cov raj yuav tsum tau hloov kho kom zoo rau cov khoom siv hauv chav kuaj kom ntseeg tau tias kev ua haujlwm du thiab cov txiaj ntsig txhim khu kev qha:

• Kev sib raug zoo ntawm centrifugalCov raj siv rau centrifugation yuav tsum muaj zog kom tiv taus cov zog centrifugal ntawm qhov ceev siab.
• Kev sib xws ntawm kev siv tshuabRau cov kev sim uas xav tau kev siv cov neeg hlau pipetting, cov tshuab faib tshuaj tsis siv neeg, thiab lwm yam, yuav tsum siv cov qhov loj me ntawm cov raj txheem.

5. Tus nqi thiab kev ruaj khov

Kev tswj hwm tus nqi thiab kev siv cov peev txheej kom tsim nyog kuj yog ib qho tseem ceeb uas yuav tsum xav txog thaum ua kom tau raws li qhov xav tau ntawm kev sim:

• Siv ib zaug xwb piv rau siv dua: cov raj pov tseg yooj yim rau kev tswj hwm thiab zam kev ua qias tuaj, tsim nyog rau kev sim siab; cov raj iav rov siv tau yog tsim rau kev sim yooj yim nrog cov peev nyiaj tsawg.
• Kev ntsuas kev yuav khoom: kev yuav khoom ntau ntau tuaj yeem txo tus nqi ntawm ib chav, tsim nyog rau cov haujlwm ntev, loj; cov lus qhia me me, cov lus qhia tshwj xeeb yog hloov pauv tau yooj yim dua tab sis tus nqi kuj siab.

Cov Lus Qhia rau Cov Xwm Txheej Siv Feem Ntau

Raws li qhov xav tau tshwj xeeb ntawm ntau hom kev sim, cov raj xa dej pov tseg hauv qab no raug pom zoo rau ntau qhov xwm txheej siv, uas tsom mus rau kev txhim kho kev ua haujlwm zoo thiab kev ntseeg siab ntawm kev sim:

1. Kev cog qoob loo ntawm tes

• Hom pom zoo: cov raj polypropylene sterile nrog cov hau cartridge breathable
• Vim li cas: Cov khoom siv polypropylene muaj kev tsis sib haum xeeb zoo thiab biocompatibility, tsim nyog rau cov kua kab lis kev cai ntawm tes. Lub hau cartridge tuaj yeem ua tiav kev sib pauv roj zoo, tiv thaiv kev kis kab mob microbial, thiab ua tau raws li qhov chaw ib puag ncig roj uas xav tau rau kev loj hlob ntawm tes.

2. Kev paub txog PCR/qPCR molecular

• Cov hom pom zooCov raj PCR tshwj xeeb uas tsis muaj nuclease, tsis muaj pyrogen lossis cov raj microcentrifuge
• Vim li cas: Cov raj polypropylene purity siab, tom qab kev kho mob nruj, tuaj yeem tiv thaiv kev puas tsuaj lossis kev ua qias tuaj ntawm cov qauv, kom ntseeg tau tias muaj txiaj ntsig zoo thiab txhim khu kev qha. Kev tsim qauv phab ntsa nyias raug pom zoo kom txhim kho kev ua haujlwm ntawm kev hloov pauv cua sov.

3. Khaws cia rau qhov kub tsawg

• Hom Pom ZooCov Tubing Polypropylene Uas Tiv Thaiv Kub Tsawg Nrog Kev Tsim Kho Kom Tsis Txhob Khov Thiab Lub hau kaw
• Vim li casCov raj no siv tau los khaws cov qauv rau lub sijhawm ntev ntawm -80 ° C lossis txawm tias hauv cov kua nitrogen, thiab cov qauv tshwj xeeb tiv thaiv lub raj kom tsis txhob tawg thiab cov qauv xau. Haum rau kev khaws cia ntev ntawm cov kab cell, cov qauv ntshav, cov protein lossis nucleic acids.

4. Lub tshuab Centrifuge

• Cov hom pom zoo: cov raj polypropylene uas ua haujlwm siab, tsim puag ncig lossis conical hauv qab, nrog rau cov rotors centrifuge
• Vim li cas: Cov raj PP muaj kev tiv thaiv centrifugal zoo thiab tuaj yeem tiv taus lub zog centrifugal siab yam tsis muaj kev hloov pauv lossis tawg. Lub hauv qab conical pab sau cov hlwb lossis precipitates hauv nruab nrab thiab ua rau kom rov zoo dua.

Cov Yuam Kev Feem Ntau Thiab Cov Txoj Kev Kom Tsis Txhob Ua Li Ntawd

Nyob rau hauv kev xyaum ua, kev sim ua tsis tiav vim yog kev xaiv cov raj xa dej tsis raug tshwm sim los ntawm ib lub sijhawm mus rau ib lub sijhawm. Muaj ntau qhov yuam kev thiab cov lus qhia rau kev daws teeb meem tau teev tseg hauv qab no rau cov kws tshawb fawb siv:

1. Siv cov khoom siv uas tsis kub thiab tsis kub heev rau kev ua kom tsis txhob kub dhau